Device

Part:BBa_K392042

Designed by: Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi   Group: iGEM10_Osaka   (2010-10-25)


exoglucanase/CBM secretion testing construct for E. coli

Device containing C. fermi exoglucanase Cex fused with cellulose-binding motif CM10; E. coli PelB export tag attached upstream to enable secretion of protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 821
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 454
    Illegal NgoMIV site found at 827
    Illegal NgoMIV site found at 1329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 874
    Illegal SapI.rc site found at 957


[edit]
Categories
Parameters
None